《植物生理学报》 2016, 52(12): 1872-1876
通信作者：谷巍；E-mail: guwei9926@126.com
摘 要：
本文模拟卷柏复苏过程, 检测在不同脱水和复水时间条件下卷柏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性、丙二醛(MDA)含量、膜透性及海藻糖含量, 研究卷柏复苏过程中抗氧化系统响应机制及海藻糖变化规律, 并探讨其复苏机理。研究结果表明: 卷柏在含水量极低的情况下, 通过复苏作用可基本恢复至初始状态; SOD、CAT和POD活性, MDA含量以及细胞膜透性均随着脱水时间的延长而上升, 随着复水时间的延长而下降; 海藻糖含量亦随着脱水时间的延长而增加, 随着复水时间的延长而减少。卷柏抗氧化系统在复苏过程中启动其保护酶防御机制抵抗水分胁迫, 而海藻糖在卷柏保护生物分子过程中发挥着重要作用。关键词：卷柏; 复苏; 抗氧化系统; 海藻糖
收稿：2016-09-09 修定：2016-10-27
资助：江苏省中药资源产业化过程协同创新中心(ZDXMHT-1-5)、国家自然科学基金(81102772)和江苏省高校优势学科建设工程(ysxk-2014)。
Corresponding author: GU Wei; E-mail: guwei9926@126.com
Abstract:
To study the antioxidative system responses mechanism and the change pattern of trehalose content during the resurrection process of Selaginella tamariscina (Beauv.), we measured the enzyme activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), the content of malondialdehyde (MDA) and trehalose, and the membrane permeability during the different dehydration and recovery water time. The results showed S. tamariscina (Beauv.) spring can be recovered to the initial state in the case of low water content through the resurrection process. The enzyme activity of SOD, CAT and POD, the content of MDA and the cell membrane permeability were all increased associated with the extension of dehydration time, and declined associated with the extension of recovery water time. The change pattern of trehalose content was similar as the antioxidant enzymes and membrane system during this process. The antioxidative system launched its protective enzyme defense mechanism to resist water stress in the resurrection process of S. tamariscina (Beauv.) spring. Trehalose play an important role in the protection of resurrection process of S. tamariscina (Beauv.) spring.Key words: Selaginella tamariscina (Beauv.) spring; resurrection; antioxidative system; trehalose
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